K-Skin is a kit of human skin that is ready for use, and designed for absorption, irritation and skin
corrosion in vitro tests. The kit contains a circular medical polymer insert made by high precision
thermoplastic injection. K-Skin is the ideal solution in terms of:

Timesaving: K-Skin is compatible with Franz cells (diameter 9 mm - 20 mm) without any
preparation and in this way bypasses skin membrane preparation and characterisation
processes (fat removal, preparing skin samples of the appropriate diameter and thickness,
and integrity tests…). On average, K-Skin reduces laboratory preparation time by 45 minutes
for each biopsy.


Test optimisation: thanks to the standardisation of the inserts in our kits, the membranes
under study have a homogeneous tension that is representative of the prevailing in vivo
tension, leading to more thorough analysis and methods of comparison. The tension
coefficient is calculated by printing of the skin explants.


Provision: PROVISKIN provides a wide variety of human skin samples (e.g. hip plasty,
abdominoplasty,pelvic plasty…), both frozen and fresh, characterised according to your
needs (e.g. phototype, donor sex and hair density). In addition, our partners network
guarantees your deliveries of fresh explants come from a healthcare facility near your
laboratory. (Biopsies without K-Skin are also possible; simply indicate your preference on
the order form)

 

K-Skin : homogenisation and reproduction of the in vivo tension

 

 

One fundamental characteristic that influences ex vivo studies is the tension of skin samples. This is particularly important in studies where the tension or thickness of the skin membrane is a parameter (e.g. percutaneous absorption studies). The picture below shows printings of skin samples with tension applied by an operator a) and within K-Skin b). After retraction the membrane has a relative surface area bigger than that in vivo, due to micro-relief and micro-wrinkle.  During studies conducted at different hospitals facilities we determined that the retraction coefficient is on average of 32%. By applying a specific pressure on the edge of the membrane K-Skin enables homogenisation and reproduction of the in vivo tension.